Preparation of blood smear archives medical students corner. Label precleaned slides preferably frostedend with patients name or other identifier, date and time of collection. Comparison of five methods for preparing blood smears. Always start with room temperature, well mixed, clot free, edta anticoagulated blood. A properly performed and analyzed blood smear is the most informative of all hematologic tests, allowing examination of erythrocytes. Peripheral blood smear test purpose, procedure, results. Ensure you are accurately identifying cells at the microscope with clinical hematology atlas, 5th edition. In the latter case, the choice of ethylenediaminetetraacetic acid edta is advisable with respect to other anticoagulants e. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. The optimal blood smear is a single layer of dispersed cells that accurately represents the distribution of cells in whole blood. Request pdf guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear.
To illustrate the simplicity of the method of making and staining slide smears, let me say that the physicians in the receiving ward of the cook county hospital, working two at a time, and diagnosing from 100 to 200 cases daily, find time to make, stain and examine blood smears by this method to help in differentiating typhoid, malaria, and. The peripheral blood film remains a very useful tool in hematological assessment. Choose from 500 different sets of preparing staining a blood smear flashcards on quizlet. The slides should be dried as quickly as possible to prevent. May, 2016 preparation of blood smear wedge method 1 a small drop of blood 23 mm in diameter is placed in the center line about 1 cm away from one end of a glass slide typical size of slide is 75. Smears made from edtaanticoagulated blood should be made. Peripheral blood test your knowledge below is a slide at high magnification under an oil immersion lens. When first learning, try multiple methods to determine which process works best for you.
A series of five slides was prepared from each sample of blood. Pdf the peripheral blood film pbf is a laboratory workup that. An illustrated guide to the morphology of blood cells, atlas of canine and feline peripheral blood smears covers patient assessment for common hematologic disorders and diseases in dogs and cats. Each person may have their own preferencemethods for success. The drop should be in the center line of the glass slide. Edta will show slight changes if the smear is prepared within an hour. Place a small drop of blood, or one side about 12 cm from one end.
Thin and thick blood smears should be prepared immediately or within 1 hour after collection. As soon as the drop of blood is placed on the glass slide, the smear. Samples collected with other anticoagulant will distort the cells. The wedge smear is a convenient and commonly used technique for. Mar 18, 20 how to properly inoculate from broth to broth, broth to slant, and broth to plate tsb, nas, nap duration. Video is prepared by the faculty and students of the department. Guidelines for blood smear preparation and staining procedure for. Slide preparation can be quite laborious especially if. The use of new, clean, greasefree, unscratched slides is essential. Examination of thin blood films is important in the investigation and management. Clean slides with 70 to 90% alcohol and allow to dry. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge.
The volume of blood and the size of the thick film detailed in the procedure below have been calculated based on. A small drop of blood may be insufficient to prepare a slide of sufficient. Preparation and staining methods for blood and bone marrow. Peripheral blood smear can be used for est imation of. A preparation for microscopic examination made by spreading a drop of fluid, such as blood, across a slide and using the edge of another slide to leave a uniform film. Thin smears are made as per routine hematology differentials. If too thick, the cells appear too round to properly identify. Preparation of blood smears in order to reduce the number of unacceptable smears received, marshfield labs recommends the following procedure to be used in preparation of blood smears. Three mechanical devices for preparing blood smears were compared to the traditional coverslip and manual wedge methods. Blood smear is prepared, stained with leishmans stain and cells are identified under oil immersion lens. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass. A suitably sized droplet and a steady motion with the top spreading slide are essential for good blood film preparation, creating a defined and extensive feathered area.
Prepares an acceptable blood smear for routine hematological studies using proper blood drop size. The smear can then be stained as soon as it is dry, generally within 2030 minutes of smear preparation. Certain cell types can be damaged easily by the blood. Pdf clinical hematology atlas download pdf free ebook. Peripheral blood or potassium edta anticoagulated blood 12 mg edta1 ml blood may be used. It is not appropriate to use the same edge of a slide for more than a single film.
Blood smears are made using fresh blood or edta blood. Making a quality blood smear although there are several techniques described for making blood smears, most people use the wedge or push technique. Glass cover slips are mounted on all blood smears to prevent damage to smear during examination, cleaning, handling and storage. Introduction to peripheral blood smear examination clinical. Blood smear preparation properly prepared blood smears are essential to accurately assess the patients status. An introduction to haematology and blood smear preparation. This new methylene bluestained blood smear shows large numbers of heavily stained reticulo.
Over 1,000 fullcolor photomicrographs depict abnormalities within each blood cell line, with multiple pictures of each morphologic abnormality and. These are the same cases that you studied in the two labs the normal smear is in there as well. Since only minimal amounts of blood are needed for the preparation of blood smears, it can be used to characterize thrombocytopenia in pediatric patients and even newborns further. Always start with room temperature, wellmixed, clot free, edta anticoagulated blood heparin is used for some exotic species. Leishmans stain maygrunwaldgiemsa stain jenners stain wrights stain principle. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with. A carefully prepared blood smear is vital for an accurate differential count. If preparation is not fixed or sticked over the slide, the cells are washed off during staining. Find a good area of the slide then scan at 25x or 50x examining cells at 100x as needed. An extensive study was undertaken to compare five methods of blood smear preparation. In principle, it is based on visualizing alterations in the distribution of proteins, which result from specific genetic mutations by using monoclonal antibodies. Either capillary blood, obtained by fingerprick, or anticoagulated blood can be used for the preparation of blood smears.
Useful smears are shown in figure 3, a and b, even though one of the smears fig. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Note that the diameter of the red blood cell is similar to the diameter of the small lymphocyte nucleus. Preparation of peripheral blood smear and staining procedure. Blood smear preparation pdf download 3d39b66ab9 this material enables the simple and lowcost preparation of samples and does not compromise the morphologic and staining characteristics of the parasites or blood cellsthis ensured that the entire length of the smear on the acetate was simultaneously stainedor its licensors or contributorssecond. Jan 17, 2017 the video shows the procedure for preparation of a peripheral blood smear and its staining prior to observation under a microscope. Prior to staining, fix the material to be observed over the slide.
Alternately, edta anticoagulated blood may be used if the smear is made within one. Parasites smear giemsa stain, blood arup lab test directory. Blood smear synonyms, blood smear pronunciation, blood smear translation, english dictionary definition of blood smear. Diagnosis of inherited platelet disorders on a blood smear. The following guidelines have been developed to assist the clinician in proper technique, slide preparation, and sample shipping. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. Therefore it need a free signup process to obtain the book. Introduction to peripheral blood smear examination oncohema key. Preparation technique, therefore, is of the utmost importance in the blood cell differential. State the two samples which may be used to prepare a blood smear. Blood smear definition of blood smear by medical dictionary. Blood smears can be made from the blood remaining in the needle by pushing a small drop of blood onto the slide with the last evacuated tube. If the smear is made too thin, there is a chance the larger cells will be collected at the thin edge.
If the blood has been refrigerated, allow it to return. Blood smear article about blood smear by the free dictionary. White blood cells or leukocytes are primarily involved in defense from invasion and infection. Thick smears are made by dropping 10 to 20 l of blood onto a slide and spreading it into a dimesized area with a glass, wooden, or plastic applicator. If the slide has been stored in spirit, ensure that all the spirit has been thoroughly washed away with water and the slide well dried with a soft, lint free cloth or paper tissues. Norgan ap, arguello he, sloan lm, fernholz ec, pritt bs. Ppt blood smear preparation and staining powerpoint. Prepare a second smear by repeating steps 35, using the edge of the first slide to spread a drop of blood on the second slide. Slides should not be left uncovered on top of the counter. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Examination of thin blood films is important in the investigation and management of anaemia, infections, and other conditions which produce.
There are multiple methods to blood smear preparation. Blood smear preparation and staining 1 blood smear preparation and staining. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of results provided to the physician. When carrying out a complete blood count, preparation and evaluation of a blood smear should always be performed alongside. To illustrate the simplicity of the method of making and staining slide smears, let me say that the physicians in the receiving ward of the cook county hospital, working two at a time, and diagnosing from 100 to 200 cases daily, find time to make, stain and examine bloodsmears by this method to help in differentiating typhoid, malaria, and.
Scroll left and right to see various peripheral blood components. The video shows the procedure for preparation of a peripheral blood smear and its staining prior to observation under a microscope. Perform a capillary puncture using aseptic technique. Blood smear definition of blood smear by the free dictionary. Welcome,you are looking at books for reading, the the smear, you will able to read or download in pdf or epub books and notice some of author may have lock the live reading for some of country. Atlas of canine and feline peripheral blood smears. The most common method of blood smear preparation is the wedge technique, in which a drop of blood is placed near the end of one glass slide.
Learn preparing staining a blood smear with free interactive flashcards. Free and intracellular bacteria on peripheral blood smears. How to properly inoculate from broth to broth, broth to slant, and broth to plate tsb, nas, nap duration. The purpose of preparation of blood smear blood film is to study the morphology of rbcs, differential leukocyte count and reticulocyte count.
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